Description
ORFeome v 8.1 Human ORF Clone Collection for Gene Overexpression
Arrayed Collection of Human ORF Clones for functional studies of the Human Proteome
• 12,071 individually arrayed ORF clones representing 11,016 human genes
• Full-length, well annotated and sequence verified ORFs
• ORFs without stop codons
• Constructed in Gateway® Entry vector enabling transfer into any type of Gateway® compatible destination vector
MGC premier human ORFeome version 8.1 (hORFeome v8.1) is the most up to date version of the human ORFeome developed by the Center for Cancer Systems Biology (CCSB). This collection is derived from the Mammalian Gene Collection (MGC) and contains 12,071 clonally-derived ORFs that represent 11,016 human genes. The ORF clones in this library contain the coding sequences located between the initiation and termination codons, excluding the 5′ and 3′ mRNA untranslated regions. Each ORF clone in the collection has been verified by next
generation sequencing and is provided in the Gateway adapted entry vector pDONR223 for fast and convenient transfer to any compatible expression vector. The termination codon has been removed allowing to add a 3′ tag to their ORF of interest if desired.
hORFeome v8.1 represents a central resource of single-colony, fully-sequenced cloned human ORFs which can be readily transferred to Gateway compatible destination expression vectors for various functional proteomics studies. This set of ORFs ranges in size from 75 to more than 10,000 base pairs, and contains over 1,000 ORFs from genes with multiple splice variants.
CONTENTS:
The ORFeome v 8.1 Library is delivered as 96-well glycerol stock plates (with lids) – 132 plates total. Plates are sealed with aluminum sealing tape and are shipped on dry ice.
The plates should be stored at -80C upon arrival.