Description
Prepare NGS DNA Library and capture 2.1 MB human MHC Class I region for targeted sequencing
• Integration of NGS library prep and MHC Class I capture simplifies the procedure and enhances the capture efficiency
• Covers exons, introns, 5’ regulatory regions, 3’ regulatory regions, and beyond
• Detection of SNPs, indels, and structural variants/CNVs
• Save sequencing costs by selectively capturing the human MHC Class I Region
• Index Primers for sample multiplexing included
BioDynami´s MHC Class I Library Prep and Capture Kit enables the most cost-effective targeted sequencing of 2.1 Mb of the human MHC Class I locus, including all coding and non-coding regions. The MHC Class I Library Prep & Capture Kit was developed to combine NGS library prep and MHC Class I locus capture based on the CATCH-Seq technology. The integration of library prep and MHC Class I capture not only simplifies the procedure, but also enhances the capture efficiency. Comprehensive sequencing of the entire locus enables detection of SNPs, indels, and structural variants/CNVs that are not covered by other MHC targeted sequencing reagents..
Sample multiplexing is offered as follows:
• Multiplexing Index Primers: For kit with 24 reactions (Cat. # 30014S)
• Multiplexing Unique Dual Index Primers: For kit with 96 reactions (Cat. # 30014L)