• Genome-Wide Coverage with a Single 109K Module
• Single library with ~109,000 constructs
• The promoter region of each gene is targeted by 5 sgRNAs
• All mouse protein-coding genes covered—approximately 19,000 gene targets
Genome-wide pooled library of ~109,000 sgRNA constructs cloned in the pRSG16-U6-sg-UbiC-TagRFP-2A-Puro vector each targeting the promoter region of ~19,000 protein-coding murine genes. Engineered to work with deactivated spCas9 (dCas9) complexed with an activator (e.g., dCas9-VPH) to activate gene expression.
Each gene is targeted by 5 sgRNAs, specially designed according to Horbeck, et al., eLife 2016;5:e19760 DOI: 10.7554.