NeoLNP RNA Transfection Kit for Common Cells (5.0 ml)

Description

LNP-based in vitro cell transfection of mRNA, siRNA, circRNA, and saRNA

RNA Encapsulation into LNPs (Lipid Nanoparticles) for high efficiency in vitro cell transfections

The NeoLNP™ RNA Transfection Kit for Common Cells is based on Levostar´s ready–to–use NeoLNP lipid nanoparticle (LNP) system, and is tailored for transfecting common adherent and suspension cells with various RNA species, such as mRNA, siRNA, circRNA, saRNA, and other types of RNA (e.g. sgRNA mRNA co-transfection) at ultra-high efficiencies, which is enabled by utilizing novel biodegradable lipids for a uniquely efficient transmembrane transport and endosomal escape mechanism.

• Easy to Use: Requires no specialized microfluidics equipment – LNP encapsulation occurs in a one minute simple mixing step without additional incubation
• High Encapsulation Efficiency: Achieves up to 80% encapsulation efficiency for micro–volume samples, with transfection results comparable to LNPs prepared using microfluidics
• High Transfection Efficiency: For most cell lines, transfection efficiencies exceed 90%, which surpasses industry standards by far     
• Broad Cell Compatibility: Successfully validated in a large variety of human and mouse cell types, including HEK293, HEK293T, HeLa, A549, HCT116, HT-29, SW480, MCF-7, PC-3, SK-N-SH, K562, Jurkat, THP-1, HL-60, Raji, Daudi, U937, HUVEC, SH-SY5Y, HepG2, Huh7, Caco-2, T47D, BT-474, A375, hESC, hNSC, hPBMC, NIH-3T3, L929, RAW264.7, EL4, B16-F10, CT26, MC38, 4T1, EMT6, TC-1, P815, SP2/0, Capan-2
• Extremely Low Cytotoxicity: Formulated without ethanol, using novel biodegradable lipids, and sterilized with 0.2 μm filtration

Kit Contents:

• 5.0 ml NeoLNP Reagent and 24 ml RNA Dilution Buffer
• Sufficient for 400 mRNA – or 1000 siRNA- encapsulations & cell transfections in 24-well plates

Shipping and Storage:
Shipped at room temperature (≤ 25°C). Store at 2–8°C upon receipt. Avoid freezing.