NeoLNP RNA Transfection Kit for Immune Cell Lines (5.0 ml)

Description

LNP-based in vitro immune cell line transfection of mRNA, siRNA, circRNA, and saRNA

RNA Encapsulation into LNPs (Lipid Nanoparticles) for high efficiency in vitro immune cell line transfections

The NeoLNP™ RNA Transfection Kit for Immune Cell Lines is based on Levostar´s ready–to–use NeoLNP lipid nanoparticle (LNP) system, and is tailored for transfecting immune cell lines with various RNA species, such as mRNA, siRNA, circRNA, saRNA, and other types of RNA (e.g. sgRNA mRNA co-transfection) at ultra-high efficiencies, which is enabled by utilizing novel biodegradable lipids for a uniquely efficient transmembrane transport and endosomal escape mechanism.

• Easy to Use: Requires no specialized microfluidics equipment – LNP encapsulation occurs in a one minute simple mixing step without additional incubation
• High Encapsulation Efficiency: Achieves up to 80% encapsulation efficiency for micro–volume samples, with transfection results comparable to LNPs prepared using microfluidics
• High Transfection Efficiency: For most immune cell lines, transfection efficiencies exceed 80%, which surpasses industry standards by far     
• Broad Immune Cell Line Compatibility: Successfully validated in Jurkat, THP-1, DC 2.4, U266, RAW264.7 & more
• Extremely Low Cytotoxicity: Formulated without ethanol, using novel biodegradable lipids, and sterilized with 0.2 μm filtration

Kit Contents:

• 5.0 ml NeoLNP Reagent and 24 ml RNA Dilution Buffer
• Sufficient for 240 mRNA – or 600 siRNA- encapsulations & cell transfections in 24-well plates

Shipping and Storage:
Shipped at room temperature (≤ 25°C). Store at 2–8°C upon receipt. Avoid freezing.