Fast and convenient High Quality NGS Library Prep for 50 ng – 500 ng Ancient DNA inputs
• Fast: Hands-on time: 10 min, Total time: 1.5 hrs
• Easy procedure
• Ready-to-use master mix
• Less reaction components
• Less magnetic beads required: Reduced more than 50%
• Guaranteed quality: Higher library conversion efficiency
• Input DNA: 50 ng to 500 ng ancient DNA
The NGS Ancient DNA Library Prep Kit (illumina platform) was developed for construction of high quality libraries using ancient DNA (50 ng – 500 ng) as input. The kit is based on single stranded DNA library preparation, which is more effective for ancient DNA samples. Highly degraded ancient DNA is difficult for the library preparation and usually results in low library conversion rate using regular library prep approach. It has been demonstrated by scientists that ancient DNA library prep using ssDNA library prep approach has significant better outcome than that of dsDNA library prep approach. The workflow of the ancient DNA kit is simple: make the libraries in two steps followed by PCR and cleanup steps. The fast and simple 1.5-hour protocol makes libraries with even coverage and low GC-bias based on BioDynami´s unique chemistry for DNA end-polishing and adaptor addition.
Library multiplexing is possible with different types of indexes.
With BioDynami’s unique DNA library preparation technologies, the fast and simple NGS DNA Library Prep Kit allows high quality NGS library preparation to be completed in 1.5 hours with only 10 minutes of hands-on time.
Standard beads purification can be used to clean the constructed libraries after the library prep and subsequent PCR amplification step. The optimized beads purification step not only reduces the working time, but also decreases more than half of the beads amount needed.
Different Kit versions are available as follows:
Non-index (Cat.# 30084): Libraries do not have index.
Index (Cat.# 30085): Each of our index primers contains a unique barcode sequence with 6 bases that can be used to identify libraries. Library multiplexing up to 48 samples is possible.
Unique dual index (Cat.# 30086): Library multiplexing up to 96 samples is possible with unique dual indexes. We have developed a 4-Base Difference Index System. The system allows us to make indexes that have at least 4 bases different from each other in the 8 bases index length. Our unique dual indexing primers remove sequencing errors such as index hopping, index cross-contamination, mis-assignment of reads, amplification errors, and de-multiplexing errors. The primer set includes 96 pre-mixed unique pairs of i5 and i7 index primers in a 96-well plate