NGS of DNA from CRISPR Screen, >100M reads

Description

Full Service for samples from your CRISPR library screen

If you are running lentiviral gene screens with a Cellecta CRISPR sgRNA  libraries, just provide genomic DNA for each time point or treatment condition (one sample), and we will provide you with a spreadsheet enumerating the read number for each guide of the library

Service description:

– sgRNA amplification
– NGS-library preparation
– NGS on the Illumina NextSeq or HiSeq platform (100M reads)
– Deconvolution
– Enumeration of shRNA counts from raw sequencing data including statistical report

Additional bioinformatics and pathway analysis are available on request.

You can also send cells or tissue samples obtained in your screens and Cellecta will perform the genomic DNA extraction for an additional fee.

Sequencing is provided not only for Cellecta libraries but also similar non-Cellecta CRISPR libraries, such as GeCKO, Brunello, Brie library, etc. Cellecta have extensive experience in designing primer and running NGS on a large variety of pooled libraries for various types of genetic screens.

Bulk prices for multiple samples are available. Please use our service request form to contact us for more details.