If you are running lentiviral gene screens with a Cellecta CRISPR shRNA (or sgRNA/barcode cell labeling) library, just provide genomic DNA for each time point or treatment condition (one sample), and we will provide you with a spreadsheet enumerating the read number for each of the library elements. Cellecta Genetic Screens Sequencing services include shRNA barcode amplification, NGS performance on the Illumina HiSeq, deconvolution, and enumeration of shRNA barcode counts from raw sequencing data. Additional bioinformatics and pathway analysis are available on request.
You can also send cells or tissue samples obtained in your screens and Cellecta will perform the genomic DNA extraction for an additional fee – please contact us for a quotation. Bulk prices for providing multiple samples are available – please ask us for a quotation.