PCR-free NGS DNA Library Prep Kit (Illumina), with indexed adaptors
2.995,00 €
Description
Fast and convenient High Quality PCR free NGS Library Prep for 100 ng – 1 ug DNA inputs
• Fast: Hands-on time: 5 min, Total time: 1 hrs
• Easy procedure
• Ready-to-use master mix
• Less reaction components
• Less magnetic beads required: Reduced more than 50%
• Guaranteed quality: Higher library conversion efficiency
• Input DNA: From 100 ng to 1 ug
The NGS PCR free DNA Library Prep Kit was developed for construction of DNA libraries for next generation sequencing (illumina platform). The kit adds 3′-dT-tailed library adapters to both ends of DNA fragments efficiently. The kit uses double strand DNA fragments (blunt and/or sticky) as input DNA for NGS library construction, and is compatible with DNA fragments generated from both enzymatic methods (e.g. BioDynami DNA fragmentation enzymes.) and physical methods (sonication, nebulization etc.). The kit has high library conversion efficiency with 100 ng – 1 ug DNA input from various sources. Standard beads purification can be used to clean the constructed libraries after the library prep and subsequent PCR amplification step. The optimized beads purification step not only reduces the working time, but also decreases more than half of the beads amount needed.
PCR amplification is usually a standard step for library preparation of Next-Generation Sequencing. PCR is used to amplify fully ligated DNA fragments and to add index information to the libraries for pooling.
However, PCR introduces uneven amplification of DNA in some DNA regions with extreme GC-contents and secondary structures. This bias can cause very low sequencing coverage in such regions. DNA sequencing in these regions is still a huge challenge.
PCR-free library prep can reduce library bias and minimize sequencing gaps. The sequencing data from PCR-free library samples have even genomic coverage with few gaps and better depth in GC-rich regions. BioDynami´s PCR-free NGS kit offers optimal coverage in the regions that are traditionally difficult, such as high-GC content regions, low-GC content regions, and repetitive sequence regions.
Two index types are available for the kit:
Non-index (Cat.# 30040): Libraries do not have index.
Index (Cat.# 30041): Each library contains one i5 index and one i7 index. Library multiplexing up to 96 samples is possible
