Phi29 DNA Polymerase

Description

Phi29 DNA Polymerase – for Whole Genome Amplification by MDA, Rolling Circle Amplification, Sequencing and Cell-Free Cloning

• Recombinant highly purified, tag free, and cost-effective Phi29 DNA Polymerase
• Extremely active: Highly processive mesophilic enzyme (up to 70kb) with exceptionally strong strand displacement activity, ideal for isothermal amplification of minute DNA amounts
•  3’→5′ proofreading exonuclease function ensures high-fidelity amplification

TriAltus’ extremely pure, tag-free Phi29 DNA polymerase is a highly processive, mesophilic enzyme from Bacillus subtilis phage phi29, known for its exceptionally strong strand displacement activity and high processivity enabling the synthesis of long DNA fragments up to 70 kb. Its robust 3’→5′ proofreading exonuclease function ensures high-fidelity amplification, making it ideal for applications like whole genome amplification and DNA sequencing preparation.

SPECIFICATIONS

PURITY | >95% (SDS-PAGE gel analysis)

ENZYME SOURCE | Phi29 DNA polymerase (from Bacillus subtilis phage phi29) is expressed in E. coli and is purified with TriAltus Bio´s proprietary CL7 / CLīM technology. The enzyme is provided tag-free.

QUANTITY | 10,000 Units  (One unit is defined as the amount of enzyme required to convert 0.5 pmol dNTPs to acid-insoluble material in 10 min at 30°C)

STORAGE/SHIPPING CONCENTRATION | 10 U/ul 

STORAGE BUFFER | 10 mM Tris-Cl pH 7.4, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 0.5% Tween® 20, 0.5% IGEPAL® CA-630, 50% glycerol

RECOMMENDED STORAGE CONDITIONS | -20°C

EXPIRATION | 6 months from receipt when stored as directed. Avoid repeated freeze/thaw cycles.