Description
Generate WGS libraries for any genome size from 50 Mb to 5 Gb or prepare Metagenomics libraries
• 12 paired end TELL-Seq WGS libraries from samples with small genomes (50 Mb to 200 Mb) or
• 6 paired end TELL-Seq WGS libraries from samples with medium sized genomes (200 Mb to 1 Gb) or
• 4 paired end TELL-Seq WGS libraries from samples with large sized genomes (1 Gb to 5 Gb) or
• 4 paired end TELL-Seq Metagenomics libraries from microbiome samples (2.5 – 5 ng HMW genomic DNA)
Box 1 contains all necessary TELL-Seq enzymes and incubation buffers (Storage at minus 20 C).
Box 2 contains > 24 Million different TELL-Seq barcoding beads and stop & wash buffers (Storage at plus 4 C).
TELL-Seq: Long-Read Sequencing for All
• Up to 2o0 kb sequence length on Illumina by barcode linked reads
• Ultralow DNA Input: 0.5 to 5 ng HMW gDNA depending on genome size
• Simple single tube protocol – sequencing ready library in 3 hours
• Easy to automate for high throughput applications
• Free TELL-Seq software packages available for performing data analysis yourself
The TELL-Seq WGS library prep kit uses the highly innovative Transposase Enzyme Linked Long-read Sequencing (TELL-Seq™) technology ( Chen et al.,Genome Research, June 2020 ) for preparing a paired-end library to generate barcode linked highly accurate reads from an Illumina® sequencing system. Coupled with free TELL-Seq analysis software, the linked reads can be easily used for the following applications and more:
• de novo genome assembly
• Whole genome haplotype phasing
• Genome wide structural variant calling
- High resolution Metagenomics-Assembled Genomes (MAGs)
- Highly accurate species identification & abundance quantification
- Discovery of new microbial species & strains and their genomes