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Sortase A, Heptamutant Protein

495,00 

Quantity: 50 ug

    Please contact us for a quotation

     


    Description

    Recombinant Sortase A, Heptamutant Protein

    • Recombinant Staphylococcus aureus Sortase A, Heptamutant
    • Highly purified and extremely active (calcium independent transpeptidase)
    • For linkage of proteins with LPETGX motif to other proteins/molecules with glycine or aminomethylene motif

    Staphylococcus aureus Sortase A, Heptamutant is a calcium-independent Sortase A variant with seven mutations (P94R, D160N, D165A, Y187L, E189R, K190E, K196T).

    This transpeptidase cleaves between threonine and glycine in an LPXTG motif, enabling precise, site-specific linkage of proteins with a C-terminal LPETGX motif to those with an oligoglycine or aminomethylene motif. Staphylococcal Sortase A is a bacterial transpeptidase that covalently attaches proteins to the bacterial cell wall by cleaving between threonine and glycine at an LPXTG recognition motif and catalyzes the formation of an amide bond between the carboxyl-group of threonine and the amino-group of the cell-wall peptidoglycan. This chemistry has been exploited as a molecular “stapler” to site-specifically link proteins with the C-terminal LPETGX motif to other proteins or molecules possessing a glycine or aminomethylene motif. This reaction is applicable to proteins in solution and on the living cell surface.

    SPECIFICATIONS

    PURITY ~99% (SDS-PAGE gel analysis)

    ENZYME SOURCE | Staphylococcus aureus Sortase A, Heptamutant is expressed in E. coli with no
    residual amino acids on either the N or C terminus and is purified with CL7 / CLīM technology. The protein is provided tag-free.

    QUANTITY | 50 ug

    STORAGE/SHIPPING CONCENTRATION | 5 mg/mL

    STORAGE BUFFER | 17.4 mM Tris HCl pH 8.0, 0.43 M NaCl, 20% glycerol

    RECOMMENDED STORAGE CONDITIONS | -80°C

    EXPIRATION | 6 months from receipt when stored as directed. Avoid repeated freeze/thaw cycles.

    Shipping Conditions: Dry Ice
    Storage Temperature: -80C