The activated Im7 resin (crosslinked agarose 4B beads) contains a highly specific, resilient Im7 domain that binds the CL7 tag fused to target proteins. Following cleavage with the included PreScission Protease, the target protein releases from the Im7-bound CL7.
You receive 1 ml resin as a 50% suspension (2 ml slurry volume), pre-loaded in a gravity column, and 1 mg PreScission Protease. The starter kit also includes a CL7 tag expression vector of your choice, please let us know the plasmid number when placing your order.
• In a single purification step you receive purified proteins that exhibit high yield, high purity, and a high activity level.
• Purify any CL7-tagged protein including membrane and multisubunit proteins.
• The Im7 resin is reusable with no observable loss in capacity.
• The Im7 resin retains a 1:1 molar binding ratio after multiple reactivations in a variety of conditions, including various salt and detergent concentrations.
Activated lm7 is also available crosslinked to agarose 6B beads, which is the standard resin format performing at a faster flow rate. Agarose 4B beads have a larger pore size than 6B resulting in higher binding capacities, which is favourable when purifying large proteins.