T7 High Efficiency In Vitro Transcription Kit (Low dsRNA)

Description

Complete High Efficiency and High Purity IVT Kit

• Very high transcription yield
• High purity of transcribed product with extremely low dsRNA contamination
• Cap analogs can be added for producing capped mRNA by co-transcription, or the produced mRNA might be capped in a subsequent reaction step

The T7 High-Efficiency Transcription Kit is optimized for transcription reaction systems across different template and nucleotide types (including N1-Me-pUTP) . This kit enables efficient production of very high yields of mRNA from DNA templates in vitro.Using T7 promoter‑specific RNA polymerase, the kit transcribes high-yield single-stranded RNA from plasmids or PCR products containing the T7 promoter sequence, using natural or modified nucleotides as substrates. By including Cap0, Cap1, or other cap analogs in the reaction, capped full‑length mRNA can be synthesized directly via co‑transcription, or the resulting mRNA can be capped subsequently using Vaccinia Capping Enzyme or mRNA Cap 2´-O-Methyltransferase.

An input of 1 μg of linearized template DNA yields >> 200 μg of mRNA and the kit can support the production of mRNA in mg to gram scales

Kit Contents (sufficient for 50 IVT Reactions):

ATP Solution(100 mM) 100 μL
GTP Solution(100 mM)  100 μL
CTP Solution(100 mM)  100 μL
UTP Solution(100 mM) 100 μL
T7 RNA Polymerase BP-100 μL
RNase Inhibitor(40 U/μL) 100 μL
Inorganic Pyrophosphatase(0.1 U/μL) 100 μL
10×Transcription Buffer 100 μL
Control Template 5 μL
Precipitation Reagent 1 mL
DNase I (2 U/μL) 50 μL
10×DNase I Reaction Buffer 100 μL
RNase-free ddH2O 1 mL