TELL-Seq Library Prep Kit – Eukaryotic Genomes and Metagenomics (STD4)

Description

Generate WGS libraries for any genome size from 50 Mb to 5 Gb or prepare Metagenomics libraries

• 12 paired end TELL-Seq WGS libraries from samples with small genomes (50 Mb to 200 Mb) or
• 6 paired end TELL-Seq WGS libraries from samples with medium sized genomes (200 Mb to 1 Gb) or
• 4 paired end TELL-Seq WGS libraries from samples with large sized genomes (1 Gb to 5 Gb) or

• 4 paired end TELL-Seq Metagenomics libraries from microbiome samples (2.5 – 5 ng HMW genomic DNA)

This complete bundle kit includes the following components:

– Cat.# 110003: Eukaryotic Genomes and Metagenomics Library Reagent Box 1 (TELL-Seq enzymes and incubation buffers, storage at minus 20 C) and Eukaryotic Genomes and Metagenomics Library Reagent Box 2 (TELL-Seq barcoding beads, storage at plus 4 C).
– Cat.# 100003, or 100009, or 100010: TELL-Seq Library Multiplex Primers 1-8, or 9-16, or 17-24 (minus 20 C)
– Cat.# 100004: TELL-Seq Illumina Sequencing Primers (minus 20 C)

All components can also be ordered individually.

TELL-Seq: Long-Read Sequencing for All

• Up to 200 kb sequence length on Illumina by barcode linked reads
• Ultralow DNA Input: 0.5 to 5 ng HMW gDNA depending on genome size
• Simple single tube protocol – sequencing ready library in 3 hours
• Easy to automate for high throughput applications
• Free TELL-Seq software packages available for performing data analysis yourself

The TELL-Seq WGS library prep kit uses the highly innovative Transposase Enzyme Linked Long-read Sequencing (TELL-Seq™) technology ( Chen et al.,Genome Research, June 2020 ) for preparing a paired-end library to generate barcode linked highly accurate reads from an Illumina® sequencing system. Coupled with free TELL-Seq analysis software, the linked reads can be easily used for the following applications and more:

•  de novo genome assembly
•  Whole genome haplotype phasing
• Genome wide structural variant calling

•  Metagenomics studies – deciphering microbial genomes in complex mixed environmental and clinical samples:

• High resolution Metagenomics-Assembled Genomes (MAGs)
• Highly accurate species identification & abundance quantification
• Discovery of new microbial species & strains and their genomes