TELL-Seq Microbial Library Prep Kit – Microbial Genomes (STD24)
2.100,00 €
Description
Generate WGS libraries for small/microbial genome sizes up to 50 Mb
Microorganisms, such as bacteria, archaea, viruses, fungi, and protists are all around us. They have a profound impact on human life and the environment. It is estimated that there are over a trillion microbial species on Earth and 99.999% of them have yet to be discovered. There are countless reasons to identify these organisms, sequence their genomes and characterize their genes and functions.
It is challenging, if not impossible, to assemble de novo microbial genomes accurately and completely with short-read NGS platforms alone. UST TELL-Seq™ Linked Read Library Prep is a novel approach that allows an Illumina platform (or other short-read sequencing platform) to generate linked-reads capable of producing de novo assemblies of microbial genomes without a long-read sequencer. The TELL-Seq™ Library Prep is simple, fast, and economical, and requires ultra-low DNA input, making TELL-Seq™ an ideal solution for microbial isolate studies. TELL-Seq™ demonstrates exceptional performance for microbial WGS, even for challenging samples or regions with high GC content.
Kit Contents:
• Reagents to prepare 24 paired end TELL-Seq WGS libraries from samples with small genomes (1 Mb to 50 Mb)
This complete bundle kit includes the following components:
– Cat.# 110001: Microbial Library Reagent Box 1 (TELL-Seq enzymes and incubation buffers, storage at minus
20 C) and Microbial Library Reagent Box 2 (TELL-Seq barcoding beads, storage at plus 4 C).
– Cat.# 100003: TELL-Seq Library Multiplex Primers 1-8 (minus 20 C)
– Cat.# 100009: TELL-Seq Library Multiplex Primers 9-16 (minus 20 C)
– Cat.# 100010: TELL-Seq Library Multiplex Primers 17-24 (minus 20 C)
– Cat.# 100004: TELL-Seq Illumina Sequencing Primers (minus 20 C)
All components can also be ordered individually.
TELL-Seq: Long-Read Sequencing for All
• Up to 200 kb sequence length on Illumina by barcode linked reads
• Ultralow DNA Input: 0.4 to 0.6 ng HMW gDNA depending on genome size
• Simple single tube protocol – sequencing ready library in 3 hours
• Easy to automate for high throughput applications
• Free TELL-Seq software packages available for performing data analysis yourself
The TELL-Seq Microbial WGS library prep kit uses the highly innovative Transposase Enzyme Linked Long-read Sequencing (TELL-Seq™) technology ( Chen et al.,Genome Research, June 2020 ) for preparing a paired-end library to generate barcode linked highly accurate reads from an Illumina® sequencing system. Coupled with free TELL-Seq analysis software, the linked reads can be easily used for de novo microbial genome assembly
