TriAltus Bioscience – High-Performance Enzymes powered by CLiM-Technology

Our supplier TriAltus Bioscience was founded to solve a persistent challenge in protein science: How to achieve high purity, high yield, and high activity in a single, efficient purification step. The company’s name, derived from the Latin Tri (three) and Altus (high), reflects this core mission. The answer to the challenge is TriAltus´ proprietary CLiM-Technology which incoporates the extremely strong affinity between a variant of the Colicin E7 DNAse (CL7) and its inhibitor, Immunity protein 7 (iM7), as published by Vassylyeva et al.

CliM offers unmatched possibilities:

• Up to 99% purity from one round of purification
• As much as 80 x higher yield than his-tag
• Delivery of tag free enzymes with extremely high activity

For this reason the lab of Nobel prize winner Jennifer Doudna is using TriAltus Bio´s CL7 tag for purifying all their discovered CRISPR enzymes (more than 100 different ones up to date). Nucleus Biotech is pleased to offer TriAltus Bio´s extremely pure and active CRISPR enzymes SpCas9, AapCas12b, and LbCas12a, and a large variety of genomic enzymes such as Phi29 DNA Polymerase and TAT-CRE Recombinase, as well as PreScission and SUMO Proteases,  and Sortase A Heptamutant, a transpeptidase enabling site-specific protein and antibody conjugation with exceptional precision in intracellular and extracellular approaches.

Do you have an enzyme in mind which you need at highest purity, yield, and activity?

Please contact us for a custom service quote – you will be amazed about the cost efficiency! Production is offered at a flexible scale from 10 mg to >100 g, and ISO13485-compliant manufacturing of diagnostic-grade proteins is available on demand.