Fast and convenient High Quality NGS Library Prep for 5 ng – 400 ng ChIP-Seq DNA inputs
• Fast: Hands-on time: 10 min, Total time: 1.5 hrs
• Easy procedure
• Ready-to-use master mix
• Less reaction components
• Less magnetic beads required: Reduced more than 50%
• Guaranteed quality: Higher library conversion efficiency
• Input DNA: 5 ng to 400 ng CHIP-Seq DNA
The NGS ChIP-Seq DNA Library Prep Kit (illumina platform) was developed for construction of high quality NGS libraries with ChIP-Seq DNA amounts from 5 ng to 400 ng. The kit allows scientists to study target sequences of epigenetic histone modifications, of transcription factors, and of other chromatin related proteins by ChIP-Seq, which combines chromatin immunoprecipitation (ChIP) with next generation sequencing of the bound and subsequently purified target sequences. The kit has high library conversion efficiency with as little as 5 ng ChIP-Seq DNA input. The fast and simple 1.5-hour protocol makes libraries with even coverage and low GC-bias based on BioDynami´s unique chemistry for DNA end-polishing and adaptor addition.
Library multiplexing is possible with different types of indexes.
With BioDynami’s unique DNA library preparation technologies, the fast and simple NGS DNA Library Prep Kit allows high quality NGS library preparation to be completed in 1.5 hours with only 10 minutes of hands-on time.
Standard beads purification can be used to clean the constructed libraries after the library prep and subsequent PCR amplification step. The optimized beads purification step not only reduces the working time, but also decreases more than half of the beads amount needed.
Different Kit versions are available as follows: