Heidelberg Biolabs – Solutions for cell-free RNA Analysis

Our new local partner Heidelberg Biolabs GmbH is a spin-off company of the German Cancer Research Center (DKFZ) founded in 2019 with the aim to commercialize advanced methods for extracellular circulating (cell-free) nucleic acids analysis.

Heidelberg Biolabs´ core technology is CATL (Capture and Amplification by Tailing and Ligation) –  an advanced know-how technology developed by a group of scientists from German Cancer Research Center who had previously explained the origin and high stability of extracellular circulating microRNAs in biofluids (Turchinovich, A., Weiz, L., Langheinz, A. & Burwinkel, B. Characterization of extracellular circulating microRNA. Nucleic Acids Res 39, 7223-7233 (2011). Turchinovich, A. & Burwinkel, B. Distinct AGO1 and AGO2 associated miRNA profiles in human cells and blood plasma. RNA Biol 9, 1066-1075 (2012). In total, developers of Heidelberg Biolabs products have more than 15 years’  research experience in the field of circulating nucleic acids, including methods development for high-throughput sequencing and RT-qPCR. Their work resulted in multiple highly cited original research reports and reviews: https://www.heidelbergbiolabs.de/resources/our-publications.html

We are pleased to announce that Heidelberg Biolabs´ full portfolio is available via Nucleus Biotech.

Heidelberg Biolabs Products and Services:

BioLiqX Small RNA-seq Kit

Based on CATL this kit was developed for Low Input Small RNA-seq and enables highly efficient unbiased NGS library preparation from picogram amounts of small RNA.

BioLiqX cf-RNA Isolation Kit

As cell-free RNA in liquid biopsy samples such as blood plasma is highly diluted, very efficient isolation procedures are required to obtain high quality input material for subsequent molecular analysis by NGS or qRT-PCR. This kit enables the isolation of highly concentrated ultrapure cf-RNA.

BioLiqX cf-RNA Isolation QC Kit

Contains all reagents and primers required for the detection of four “house-keeping” miRNAs in any RNA sample using qRT-PCR. The kit also contains synthetic cel-miR-39 for spike-in assessment of small cf-RNA isolation between samples as well as normalization of miRNA expression in biological fluids after downstream qRT-PCR

BioLiqX HS miRNA qRT-PCR Kits

Also based on Capture and Amplification by Tailing and Ligation (CATL) technology ensuring extremely high sensitivity and compatibility with the BioLiqX Small-RNA seq libraries making them ideal for quantitative validation of Small RNA-seq data

Bioinformatics Services

  • Fully customized computational analyses of massive parallel sequencing data using validated bioinformatics pipelines tailored for circulating RNA-seq and DNA-seq experiments. 
  • Comprehensive analysis of deep sequencing data (WGS, WES or panel sequencing) obtained from tumor biopsies or liquid  biopsies with the ultimate goal to identify cancer driver genes as well as lists of druggable mutations.