BioLiqX cf-RNA Isolation Kit

Description

BioLiqX cf-RNA Isolation Kit for High Efficiency Purification of cf-RNA

• Superior cf-RNA recovery as compared to phenol-free protocols
• BioLiqx Denaturation Buffer R ensures optimal recovery of highly diluted ultra short small cf-RNA and removal of contaminating DNA
• Optimized Wash Buffers for eliminating any phenol traces
• Ultra-clean spin columns for complete removal of contaminants
• Small elution volume – obtain highly concentrated ultrapure cf-RNA

The BioLiqX Cell-free RNA Isolation Kit is optimized to isolate total cf-RNA from liquid samples including blood plasma and serum. The method is based on sample denaturation with phenol-complemented BioliqX Buffer R, subsequent phase separation and spin-column purification of RNA. The protocol efficiently denatures protein-rich biological fluids and ensures the complete inactivation of RNases. Furthermore, the optimized composition of the BioliqX Lysis Buffer R enables superior recovery of highly diluted ultra-short RNA fragments and almost complete removal of the DNA during organic extraction. In addition, optimized washing buffers ensure the absence of enzyme-inhibiting traces of phenol in the RNA eluates. Finally, ultra-clean spin columns secure the absence of contaminants and allow sample elution into the minimal volume of as little as 10 μL. The whole procedure can be completed within approximately 1.5 hours and requires a hands-on time between 10-30 minutes depending on the number of samples.

Workflow of the BioLiqX cf-RNA Isolation Kit

Kit Contents

The kit is designed for for processing fifty 400 μl samples. It contains BioLiqX Buffer R (which needs to be complemented by Phenol), 50 Spin Columns, 50 Collection Tubes, 50 Nuclease-Free Tubes, RNAse-Free Water as well as ethanol-free concentrates of Washing Buffer 1 and Washing Buffer 2.

The following components are not included and have to be supplied by the user:

Safe-Lock 2 mL microcentrifuge tubes for the initial sample denaturation
Molecular biology grade phenol
Molecular biology grade absolute ethanol
Molecular biology grade chloroform

Related product:

For assessing the cf-RNA isolation efficacy we highly recommend the BioLiqX cf-RNA Isolation QC Kit which uses qRT-PCR to detect housekeeping miRNAs hsa-miR-16, hsa-miR-21, hsa-miR-451a, and cel-miR-39. The kit also contains synthetic cel-miR-39 for spike-in assessment of small cf-RNA isolation between samples as well as normalization of miRNA expression in biological fluids after downstream qRT-PCR