Research has shown that the SARS-CoV-2 virus is internalized via binding of its S protein to the host ACE2 with the help of TMPRSS2. The binding affinity of SARS-CoV-2 with ACE2, along with ACE2 and TMPRSS2 expression levels, are major determinants of viral replication rate and disease severity. The CleanPlex ACE2 & TMPRSS2 NGS panel targets all exonic and promoter regions of the two genes, and more than 70 expertly curated expression quantitative trait loci (eQTL), shown to regulate their expression.
The panel facilitates further investigations in host genome mutations related to the ACE2 binding affinity and expression of ACE2 and TMPRSS2, providing additional insights into the individual’s viral susceptibility and disease’s severity.
The ACE2/TMPRSS2 panel is based on Paragon Genomics´proprietary CleanPlex technology which is superior to other amplicon panels or approaches such as hybridization capture based enrichment.