Pooled shRNA Libraries

The Human Genome wide shRNA libraries from our partner Cellecta are are pooled barcoded lentiviral shRNA libraries constructed in third-generation lentiviral vectors, optimized for RNAi genetic screens. They are valuable tools for simultaneous screening of thousands of different shRNA molecules on a target population.

Genetic screens represent an unbiased approach to identify genes that act in specific cellular pathways. On completion of a screen, cells exhibiting desired phenotypic changes are isolated, the shRNA constructs inducing the phenotypes are recovered by PCR and identified by Next-Generation Sequencing (NGS) of shRNA-specific barcodes.

Nucleus Biotech provides different library modules covering all protein-coding genes. The libraries are offered as plasmid DNA or packaged as pseudoviral particles

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