Description
• Single library with ~55,000 constructs
• Each gene is covered by 8 shRNAs targeting functional domains and conserved transcript regions
• Cell surface marker, extracellular matrix, and DNA binding genes covered—over 6,000 gene targets
Module 3 of Cellecta´s Human Genome-Wide (hGW) shRNA Library targeting ca. 6,300 genes, including cell surface markers, extracellular matrix genes, and DNA binding genes. Cellecta´s 3-module pooled lentiviral Human Genome-wide shRNA Library cloned in pRSI16cb targets nearly all protein-encoding genes of the human genome, and each gene is targeted by 8 hairpins. The library includes shRNA-specific barcodes that provide an alternative to direct sequencing of the shRNA target sequence which is difficult to sequence due to the stem-loop structure.