NGS Services for Pooled shRNA Libraries

In addition to NGS Prep kits for samples from pooled shRNA library screens, we are also offering Cellecta´s next-generation sequencing and analysis services for researchers running their screens with pooled lentiviral shRNA libraries from Cellecta.

Take advantage of Cellecta´s extensive experience in primer design and running NGS for various types of genetic screens.

Simply provide harvested cells or genomic DNA for each time point or treatment condition, and Cellecta will provide you with a spreadsheet enumerating the counts of each shRNA in each sample.

Service description:

You provide cells, tissue or already isolated gDNA and Cellecta performs the following service:

– gDNA isolation (optional)

– shRNA-cassette amplification

– NGS on Illumina platform: 50M or 100M reads

– Deconvolution

– Enumeration of shRNA counts from raw sequencing data

– A comprehensive NGS data report will be provided

NGS library prep services are also available for all Cellecta sgRNA-,and Barcode-Library  screens as well as for GecKO and Brunello sgRNA library screens

Nucleus Biotech will be your partner in organizing the sequencing service for samples from your genetic shRNA screens.