Microbiome Sequencing Kits

Complete solutions for NGS of metagenomics samples

Nucleus Biotech is pleased to offer complete convenient amplicon-panel based NGS kits and analysis services for deciphering complex bacterial or fungal samples:

YouSeq´s  THE ONE 16S NGS KIT:

The World’s fastest and easiest 16S NGS Library prep kit

Create a ready to sequence 16S Library for the Illumina or the Ion Torrent platform in one closed tube reaction. The ONE 16S NGS kit contains all of the reagents necessary to create a ready-to-sequence NGS library in minutes. In a breakthrough kit format, the user simply performs one closed tube qPCR reaction. The variable regions V3/4 are targeted, amplified and adapters are added in a single reaction. The quantitative PCR read out simultaneously quantifies each library so they can be pooled precisely. Then a simple bead-clean completes the workflow.
After sequencing, the data can then be loaded on the YouSeq cloud for rapid analysis. A detailed report is typically returned within 15 minutes.

Celemics´ MICROBIOME and MYCOBIOME NGS KITS:

Celemics´ Metagenomic Sequencing Kits (16S rRNA V3 or V3-V4, 18S rRNA ITS1 or ITS1-2) are used for microbiome and mycobiome studies and enable differentiation and characterization of a myriad of microbial or fungal species in genomic DNA obtained from complex metagenomic samples. The 16S V3/V4 region of bacteria and archaea resp. 18S ITS1/ITS2 region of fungi is amplified by PCR. After cleaning up using CeleMag beads, indices and adapters are attached, followed by another CeleMag bead purification, library pooling, and NGS analysis on the Illumina platform. Data Analysis by Celemics is available on request.

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