Ribosome Profiling

Quantitative transcriptome wide measurement of ribosome occupancy by Ribo-seq

The eRibo Pro RiboSeq Service

⋅ High resolution maps of ribosome footprints

⋅ Detect changes in ribosome occupancy

⋅ Receive two data sets for each provided cell sample: A Ribosome Protected Fragment (RPF) Library and an RNA-Seq Library

⋅ Quantify changes in translation and transcription in response to cell treatments

Widespread application of RNA-Seq has provided substantial insight into the role of gene expression. However it fails to capture regulation at the level of translation. The ability to quantify ribosome occupancy transcriptome-wide using eRibo Pro will enable translatome studies to be applied more broadly in the context of research and in the development of therapeutics.

eRibo Pro is a proprietary ribosome profiling service that streamlines the ribosomal profiling process, from sample processing to sequencing and data analysis. The comprehensive service includes mapping of ribosome protected fragments (RPFs) and identification of differentially transcribed and translated genes with interactive reports that allow researchers to make data driven insights.

RFP Libraries capture trinucleotide periodicity from translating ribosomes

eRibo Pro provides high correlation between RPF, RNA-Seq, and RO datasets

Ribo Pro™ is an exclusively licensed method for profiling ribosome-associated RNAs and the transcriptome. Our service provider Eclipsebio is sole licensee of the Ribo-seq method covered by U.S. Patent Numbers 8,486,865, 9,284,547 and 9,677,068.                                 

Literature references for the Ribo-Seq method:

Genome-Wide Analysis in Vivo of Translation with Nucleotide Resolution Using ribosome profiling (Science 2009, Ignolia et al.)

The ribosome profiling strategy for monitoring translation in vivo by deep sequencing of ribosome-protected mRNA fragments (Nature Protocols 2012, Ignolia et al.)