eRibo Pro Service: Ribosome Profiling by Ribo-Seq
3.800,00 €
Description
Riboseq-based Ribosome Profiling Service
Send us your cells for quantitative transcriptome wide measurement of ribosome occupancy by Ribo-seq
⋅ High resolution maps of ribosome footprints
⋅ Detect changes in ribosome occupancy
⋅ Receive two data sets for each provided cell sample: A Ribosome Protected Fragment (RPF) Library and an RNA-Seq Library
⋅ Quantify changes in translation and transcription in response to cell treatments
eRibo Pro is a proprietary ribosome profiling service that streamlines the ribosomal profiling process, from sample processing to sequencing and data analysis. The comprehensive service includes mapping of ribosome protected fragments (RPFs) and identification of differentially transcribed and translated genes with interactive reports that allow researchers to make data driven insights.
Starting Material
5M Cells
70% – 80% Confluency
Deliverable: Comprehensive Data Analysis Package (Eclipsebio’s standard analysis uses human hg38, please contact us for alternate species including viral genomes) including:
RAW SEQUENCING DATA | FASTQ
Reads directly off sequencer and demultiplexed per sample for data back-up and publication
GENOME ALIGNMENTS | BAM
Reads filtered of repetitive elements, aligned, and PCR deduplicated for additional downstream analysis
COVERAGE TRACKS | BIGWIG
Normalized read coverage on positive and negative strands for visualization in a genome viewer such as IGV
DIFFERENTIAL EXPRESSION & RIBOSOME OCCUPANCY | TSV
Results and statistics table for differential gene count analysis using DESeq2 for the RPF and RNA-Seq libraries, and for the calculated ribosome occupancy. Includes log2 fold changes and p-values
eRibo SUMMARY REPORT | HTML
Report comparing libraries from ribosome associated (RPF) and RNA-Seq for a given sample and for samples from two different conditions with interactive tables and plots for publication ready graphics. Includes QC metrics, visualization of ontology enrichment, and differential genes
For an example report click here
Please contact us to describe your Ribosome Profiling project and to receive an eRibo Pro service quotation