RNA-Seq Barcode Pooled Libraries

Clonetracker XP Lentiviral Barcode Libraries

Whole cell population labeling with stable, genomically integrated, gDNA-NGS and RNA-seq sequenceable barcodes for cell tracking, lineage tracing, and quantification of descendants in mixed downstream cell or tissue cultures.

• Label large cell populations of any type by lentiviral barcode library transduction
• Assess how cell heterogeneity changes in response to drugs, differentiation, or disease progression (e.g. metastasis)
• Readout by gDNA NGS and single-cell RNA-seq, enabled by barcode placement in the 3´UTR of the resistance gene transcript

RNA-Seq Barcode Lentiviral Pooled Libraries link genetic barcode labeling of cells with changes in expression profiles. The approach provides a convenient way to identify cell variations with unique characteristics or biology, and to understand how these groups of variant cells evolve in response to drug treatment, tumor metastasis, or other conditions.
Nucleus Biotech offers CloneTracker XP Barcode Libraries, where the barcode is positioned in the 3’-UTR (or 5′-UTR) of the selection marker gene so it is expressed as part of the RNA transcript in the cells. As a result, it can be detected by RNA sequencing, as well as DNA sequencing.

Choice of ClonetrackerXP Lentiviral Barcode Libraries

You can choose from a 50M XP library, a 10M XP library, and from one to ten XP sublibrary pools each of 5M in size:

The 50M XP library is available with either RFP/Puro markers, or  RFP/ Thy 1.1 (for mouse in vivo studies)

The 10M XP library can be delivered in 4 different vectors with either red-shifted luciferase (rLuc) derived from Photinus pyralis (Ppy RE9 mutant) as a reporter for bioluminescent imaging (BLI)  /Puro, with Venus Fluorescent Protein/Puro markers, with eGFP/Blast markers, or with Puro marker only.

Up to ten 5M XP sublibraries in the RFP/Puro vector with defined barcodes are available to label several cell populations with distinct non-overlapping barcodes. To each of the ten pools library specific 14nt barcodes are assigned to enable the differentiation of the 5M pools to each other.