CleanPlex BRCA1 & BRCA2 NGS Panel

Description

The CleanPlex® BRCA1 & BRCA2 NGS Panel v3 for Illumina® or Ion Torrent™ is a multiplex PCR- based targeted sequencing assay designed to simplify the evaluation of somatic and germline variants across BRCA1 and BRCA2 genes

• High Coverage of Target Regions – Target the entire coding regions including 20 bases of padding around all targeted exons
• Sensitive Detection – Detect somatic mutations as low as 1% variant allele frequency using just 20 ng of DNA
• Fast, Streamlined Workflow – Generate sequencing-ready libraries for Illumina or Ion Torrent platforms in just 3 hours using a rapid, three-step protocol
• Superb Performance– Prepare high-quality NGS libraries with excellent on-target performance using CleanPlex Technology to enable efficient use of sequencing reads and reduce costs

The CleanPlex® BRCA1 & BRCA2 Multiplex PCR-based NGS Panel v3 – designed to simplify the evaluation of somatic and germline variants across BRCA1 and BRCA2 genes – targets all exonic regions and 20 bp of flanking intronic sequences of BRCA1 and BRCA2. Starting with just 20 ng of DNA, sequencing-ready libraries can be prepared using a streamlined workflow in just 3 hours. The panel is optimized to deliver data with high on-target performance and high coverage uniformity to ensure efficient use of sequencing reads.

Kit Contents:Multiplex PCR Primers and PCR Reagents, & CleanPlex Targeted NGS Library Preparation Kit with CleanPlex purification reagents

Additional Products which can be ordered separately:
CleanMag® Magnetic Beads – for library purification and size selection
CleanPlex® Dual-Indexed PCR Primers – for multiplexing up to 192 samples per sequencing run
CleanPlex® Single-Indexed PCR Primers for Ion Torrent