The CleanPlex CFTR NGS Panel for Illumina® or Ion Torrent™ is a multiplex PCR-based targeted resequencing assay designed to simplify the evaluation of germline variants across the CFTR gene.
• High Coverage of Target Regions – Target the entire exon and include 20 bases of padding around all targeted coding exons
• Sensitive Detection with Low Input – Detect novel or known mutations using just 10 ng of DNA
• Fast, Streamlined Workflow– Generate sequencing-ready libraries in just 3 hours using a rapid, three-step protocol
• Superb Performance – Prepare high-quality NGS libraries with excellent on-target and coverage uniformity performance using CleanPlex Technology to enable efficient use of sequencing reads and reduce costs
The CleanPlex CFTR Panel is a multiplex PCR-based targeted resequencing assay designed to simplify the evaluation of germline variants across the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) gene. The panel targets all exonic regions and flanking intronic sequences of CFTR. Compatible with just 10 ng of DNA, sequencing-ready libraries can be prepared using a streamlined workflow in just 3 hours. The panel is optimized to deliver data with high on-target performance and high coverage uniformity to ensure efficient use of sequencing reads.
Kit Contents: Multiplex PCR Primers and PCR Reagents, & CleanPlex Targeted NGS Library Preparation Kit with CleanPlex purification reagents
Additional Products which can be ordered separately:
CleanMag® Magnetic Beads – for library purification and size selection
CleanPlex® Dual-Indexed PCR Primers – for multiplexing up to 192 samples per sequencing run
CleanPlex® Single-Indexed PCR Primers for Ion Torrent