The CleanPlex® Dual-Indexed PCR Primers for Illumina® are high-quality ready-to-use PCR primers for Illumina library construction. They are compatible and designed for use with all CleanPlex Panels to construct targeted libraries for sequencing on an Illumina NGS platform. The index set comprises of i5 primers and i7 primers that form combinatorial indexing of samples for multiplexing samples per sequencing run. Samples with unique i5 and i7 combinations can be pooled and sequenced then demultiplexed for analysis.
Contents: Set B features 12 x 8 dual indexes (includes 20 tubes, yielding 96 combinations).
Sets A and B’s index sequences are mutually exclusive and can be combined for additional multiplexing needs.