CleanPlex Mitochondrial Disease NGS Panel

Description

The CleanPlex® Mitochondrial Disease NGS Panel for Illumina® or Ion Torrent™ is a multiplex PCR-based targeted sequencing assay designed to simplify the evaluation of the entire human mitochondrial genome for mutations. The panel enriches for and provides complete coverage of all 37 genes of the ~17 kb mitochondrial genome, allowing identification of important variants.

• Relevant Gene Content – Detect and investigate important variants with complete coverage of the entire human mitochondrial genome
• Fast, Streamlined Workflow – Generate sequencing-ready libraries in just 3 hours using a rapid, three-step protocol
• Superb Performance – Prepare high-quality NGS libraries with excellent on-target performance using CleanPlex Technology to enable efficient use of sequencing reads and reduce costs

The CleanPlex Mitochondrial Disease Panel is a multiplex PCR-based targeted resequencing assay designed to simplify the evaluation of the entire human mitochondrial genome for mutations. The panel enriches for and provides complete coverage of all 37 genes of the ~17 kb mitochondrial genome, allowing identification of important variants. Starting with just 2 ng of high-quality genomic DNA (1 ng per primer pool), sequencing-ready libraries can be prepared using a streamlined workflow in just 3 hours. The panel is optimized to deliver data with high on-target performance and high coverage uniformity to ensure efficient use of sequencing reads.

Kit Contents: Multiplex PCR Primers and PCR Reagents, & CleanPlex Targeted NGS Library Preparation Kit with CleanPlex purification reagents

Additional Products which can be ordered separately:
CleanMag® Magnetic Beads – for library purification and size selection
CleanPlex® Dual-Indexed PCR Primers – for multiplexing up to 192 samples per sequencing run
CleanPlex® Single-Indexed PCR Primers for Ion Torrent