Description
This kit includes 10 lentiviral constructs with barcodes inserted in the 3´UTR of the antibiotic resistance gene. After transduction and antibiotic selection of cells, barcodes can be used for DNA-Seq to estimate the number of cells present from a specifically labeled population and in addition for single cell RNA-Seq for checking transcritional activity. In this way, multiple cell populations can be labeled, mixed, growth together, and then the representative numbers of descendant cells from each population can be determined, and by scRNA-Seq you can monitor individual cells with phenotyopes of interest for changes in their expression profiles.
Each kit contains 10 x 25 ug lentiviral plasmid DNA.