Description
Track the fate of cell clones over a course of drug treatment or during differentiation, tumorigenesis or other processes. This 50M barcode lentiviral library enables you to easily label millions of cells with heritable unique 3´ UTR RNA-expressed barcodes that are readily detected in RNA-Seq and single-cell expression analysis as well as in the genomic DNA fraction. The barcode location in the 3´ UTR enables oligodT-primed cDNA synthesis.
With this CloneTracker XP library in pScribe4ThyM-RFP-Thy1.1, barcoded cells are detectable both by RFP fluorescence and by expression of Thy1.1. The Thy1.1 antigen is expressed by the barcoded constructs. This is useful for labs doing in vivo mouse studies.