DriverMap AIR Adaptive Immune Receptor Repertoire Profiling (Mouse RNA): TCR Profiling Kit, CDR3 Region (96 rxns)


Quantity: 1 kit (96 reactions)

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    Profile T-Cell Receptor (TCR) adaptive immune receptor repertoire by AIRR-seq starting from mouse total RNA

    Easy-to-run, single-day AIRR-seq assay that uses multiplex PCR-NGS technology to profile adaptive immune receptor repertoires

    • Only assay technology on the market that profiles the mouse CDR3 repertoire of all T-Cell Receptor (TCR) and B-Cell Receptor (BCR) chains in a single-tube AIRR-seq assay
    • Start from various immune sample types such as whole blood, PBMC, tissue, and FFPE samples
    • Detect and accurately quantify more AIR clonotypes by Unique Molecular Identifiers (UMIs)
    • Amplify only functional TCR/BCR mRNA isoforms, without pseudogenes, ORFs or misarranged genes

    Cellecta’s DriverMap™ AIR Adaptive Immune Receptor Repertoire Profiling assay is a next-generation targeted multiplex RT-PCR NGS technology designed for bulk expression profiling in a single assay of all functional T-Cell Receptors (TCR) and B-Cell Receptors (BCR): TRA, TRB, TRD, TRG, IGH, IGK and IGL mRNA isoforms excluding non-functional pseudogenes and ORFs.

    While the kit described here profiles the T- cell chains only, we recommend profiling both TCR and BCR repertoires together, since in most cases, T- and B-cells work synergistically in the adaptive immune response.

    Kit Description and Contents:

    • Profile all TCR chains (TRA, TRB, TRG and TRD) in a single reaction from a single sample.
    • Includes all the enzymes, reagents, and primers to prepare 96 samples ready for NGS from mouse total RNA.
    • Assay(s) can be run in a single reaction without having to run multiple expensive assays and without any specialized equipment
    • Generate interactive reports for clonotype quantitation, CDR3 length distribution, V(D)J segment usage, and isotype composition using MiXCR (MiLabs) adaptive immune receptor repertoire profiling software.

    Shipping Conditions: Blue Ice
    Storage Temperature: -20C