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Human CRISPRa Dual-sgRNA Library (UbiC-RFP-Puro) (packaged)-academia

6.250,00 

Quantity: 2 x 10^8 TU

    Please contact us for a quotation

     


    Description

    • Genome-Wide Coverage with a Single 105K Module
    • Single library with ~105,000 constructs
    • The promoter region of each gene is targeted by 5 sgRNAs in dual combinations of each of the 5 sgRNA designs enhancing gene activation
    • All human protein-coding genes covered—approximately 19,000 gene targets

    Genome-wide pooled library of ~105,000 sgRNA constructs cloned in the pRSL10-mU6-sg1-hU6-sg2-UbiC-TagRFP-2A-Puro vector targeting the promoter region of ~19,000 protein-coding human genes. Designed to work with deactivated spCas9 (dCas9) complexed with an activator (e.g., dCas9-VPH) to induce gene expression.
    The promoter region of each gene is targeted by 5 sgRNAs in dual combinations of each of the 5 sgRNA (design based on Horbeck, et al., eLife 2016;5:e19760 DOI: 10.7554). Targeting genes with dual-sgRNA constructs leads to increased gene activation.

    Shipping Conditions: Dry Ice
    Storage Temperature: -80C