Magnetic Beads Kit for miRNA Purification / tRNA Depletion

Description

miRNA Purification by depletion of tRNA from small RNA preparations

• Purification of miRNA by removing tRNA, 5S RNA, and larger RNA contaminations

• Removal of unwanted impurities such as salts, proteins, dNTPs, and detergents

The miRNA Purification/tRNA Depletion kit is developed for microRNA (miRNA) purification by removal of tRNA, 5S RNA, and larger RNA contaminations.

microRNAs are typically around 22 nucleotides long and can be used in various downstream applications relating to gene expression regulation, miRNA profiling, and functional analysis. However, miRNA isolated from commercial microRNA extraction kits usually contain contaminations of tRNA, 5S RNA, and larger RNA molecules. The contamination may result in decreased sensitivity or inconsistent data for applications such as PCR, RT-PCR, next-generation sequencing (NGS), microarray, etc.

BioDynami have developed a Solid Phase Reversible Immobilization (SPRI) beads-based technology to solve the problem. SPRI beads are well used for nucleic acid purification. The paramagnetic beads coated with carboxyl groups can reversibly bind to nucleic acid that are 100 base pairs or longer. However, with traditional SPRI beads, nucleic acids shorter than 100 bp are poorly recovered.

BioDynami´s proprietary magnetic bead technology not only overcomes the short nucleic acid recovery hurdle, but also enables effective separation of miRNA from large RNA molecules. With this technology, the kit selectively purifies miRNA and removes tRNA, 5S RNA, and larger RNA molecules. The magnetic beads can also remove impurities and unwanted components such as salts, proteins, dNTPs, and detergents.