miR-eCLIP Service: miRNA Target Identification by AGO2-eCLIP-seq
4.800,00 €
Description
miR-eCLIP based miRNA target sequencing
Send us your cells or tissue for identifying miRNA target sites by miR-eCLIP-Seq
- High resolution maps of the miRNA-mRNA interactome
- Precise mapping of direct miRNA-mRNA interactions transcriptome wide
- Reveal functional miRNA targets
- Optional enrichment of miRNAs, miRNA families, or genes for distinct target profiling
- Service also applicable to look for siRNA off-target sites
Starting Material
Cells: 20 M; UV Crosslinked
Tissue: 80 mg; Non-Crosslinked; Flash Frozen
Antibody used for eCLIP:
Eclipsebio provided AGO2 antibody
Reactive in human, mouse, rat
Deliverable: Comprehensive Data Analysis Package (Eclipsebio’s standard analysis uses human hg38, please contact us for alternate species including viral genomes) including:
RAW SEQUENCING DATA | FASTQ
Reads directly off sequencer and demultiplexed per sample for data back-up and publication
GENOME ALIGNMENTS | BAM
Unique AGO2 eCLIP sequence alignments to the genome. Reads aligned to repetitive elements were filtered out and PCR duplicates were removed.
COVERAGE TRACKS | BIGWIG
Normalized read coverage on positive and negative strands for visualization in a genome viewer such as IGV
AGO2, miRNA, & siRNA PEAKS | BED
Table listing the binding locations and relative enrichment of siRNAs and miRNAs
TARGET SUMMARY REPORT | HTML
HTML report containing plots, enriched GO terms and KEGG pathways, HOMER motif analysis, and repetitive element mapping information for enriched peaks.
For an example report click here
Please contact us to describe your miRNA target identification project and receive your miR-eCLIP service quotation