NGS Prep Kit for sgRNA Libraries in pRSG16/17-including Alignment Software

Description

Enhance the NGS output of your pooled sgRNA library screens by Cellecta´s amplicon-based NGS library prep technology

Cellecta’s NGS Library Prep Kit for sgRNA Libraries cloned into the pRSI16 and pRSI17 vectors (e.g., CRISPR Human, Mouse, Chicken, or Pig Genome Knockout Library) provides a protocol and reagents for PCR amplification and NGS sequencing of sgRNAs  from samples of your genetic screen performed with Cellecta´s CRISPR ko Libraries.

Features:

• Cost and time savings by substantially fewer PCR reactions compared to conventional low gDNA-input method
• Avoiding background by low PCR cycle number
• Optimized protocol and reagents  allows as high as 50 µg gDNA input per PCR reaction
• Unique indexing primers to multiplex up to 12 samples run in a single lane.
• Software included with the kit enables rapid and convenient analysis of the sequencing. The program demultiplexes indexed samples and aligns barcode reads from the Illumina NGS FASTQ files. The output is a spreadsheet with the counts of each barcode in each sample.

Workflow:

Deliverables:

• PCR reagents and specific primers, sufficient to process 12-48 50µg gDNA samples
• Index Primers to multiplex up to 12 samples run in a single lane
• NGS sequencing primers
• Free Data analysis software