The OmniFusion RNA Lung Cancer NGS Panel for Illumina® or Ion Torrent™ uses single primer amplification method enabling detection of more than 530 known and unknown fusions associated with non-small cell lung cancer (NSCLC)
• Identification of known and novel RNA fusions associated with lung cancer – Interrogate >530 known RNA fusions and additional novel RNA fusions using a single-primer method.
• Fast, streamlined workflow – Generate sequencing-ready libraries in just 6.5 hours using a rapid, five-step protocol from extracted RNA to sequence ready libraries.
• Excellent performance with extremely low rRNA rate – Prepare high-quality targeted NGS libraries using OmniFusion RNA Fusion Detection Technology to achieve minimal rRNA and over 90% on-target rate.
• Sensitive Detection – Can detect down to 1% variant frequency with high confidence.
The OmniFusion RNA Lung Cancer NGS Panel enables detection of more than 530 known and unknown fusions associated with non-small cell lung cancer. The OmniFusion workflow allows detection of novel fusion partners and and splicing variants. Compared to other methods such as qPCR, FISH, or Sanger sequencing, Paragon Genomics´ targeted fusion sequencing method allows robust multiplexed detection of variants using minimum sample input and a simple workflow.
The OmniFusion single-primer amplification technology uses template switching technology to add an universal sequence to the 5’ ends of RNA fragments. Combined with expertly designed targeted 3’ primers specific to acceptor genes, the process eliminates the constraint of template length, and allows higher sensitivity even with damaged RNA fragments, such as FFPE RNA. The single sided amplification method enables the detection of any mutations on the RNA fragments, including novel ones.
Kit Contents: Reagents for Reverse Transcription and PCR, Multiplex PCR Primers, & OmniFusion Targeted NGS Library Preparation Kit
Additional Products which can be ordered separately:
CleanMag® Magnetic Beads – for library purification and size selection
CleanPlex® Dual-Indexed PCR Primers – for multiplexing up to 192 samples per sequencing run