Cellecta offers Custom CRISPR sgRNA Pooled Libraries in the CROP-Seq designed vector pScribe (Datlinger et al., Nature Methods, 2017) suitable for Perturb-Seq on any single cell analysis platform. Libraries used for Perturb-seq analysis need to be relatively small and targeted. A 0.24K library featuring up to 240 sgRNAs is suitable for this purpose.
pScribe either contains an sgRNA-only expression cassette or an sgRNA-Barcode expression cassette for clonal tracking.
Cellecta offers flexibility in library formats, providing both plasmid and pre-packaged ready-to-transduce options. The availability of multiple vector backbones with different selection and fluorecence markers allows researchers to choose the most suitable setup for their experiments.
Cellecta has over 15 years extensive experience in creating high-quality pooled shRNA, barcode, and sgRNA libraries.
Note: For species other than human and mouse, a special sgRNA design fee may apply. Please contact us for more details.