PreScission Protease (PSC) or Human Rhinovirus HRV 3C Protease recognizes the sequence LEVLFQ/GP and cleaves between the glutamine (Q) and glycine (G).
Cleavage Efficiency: 20 ug protease will digest 2 mg protein with 99% efficiency at 4°C in 60 minutes.
The GST-tagged PSC protease can be used to release target proteins from TriAltus’ Im7 resin. The protease cleaves and releases the target protein from the Im7-bound CL7 tag. The final protease concentration in the eluted sample comprises ~1.5-3%. If needed, PSC Protease can be removed from the sample with a GST-trap column or via size exclusion chromatography.