Fast and convenient High Quality RNA-Seq NGS Library Prep for 3 ng – 100 ng RNA inputs
• Fast: 2-hour protocol from intact purified RNA to NGS library, 10 min hands-on-time
• Easy procedure, ready-to-use mastermixes
• Less reaction components, less magnetic beads
• Guaranteed conversion quality: High yield, uniform coverage of transcripts • Input RNA: Purified RNA from 3 ng to 100 ng
The RNA Seq Library Prep Kit was developed for construction of high quality NGS libraries for next generation sequencing (illumina platform). RNA Sequencing is a very powerful transcriptomics tool to study gene expression, transcription regulation, splicing events, mutations and variations and more. The kit needs purified RNA, e.g. rRNA depleted RNA or polyA mRNA. BioDynami´s technology provides a fast and simple workflow. RNA-Seq libraries can be generated in around 2 hours with only 10 min hands-on time. Library multiplexing is possible with different types of indexes.
Standard beads purification can be used to clean the constructed libraries after the library prep and subsequent PCR amplification step. The optimized beads purification step not only reduces the working time, but also decreases more than half of the beads amount needed.
Different Kit versions are available as follows:
Non-index (Cat.# 30055): Libraries do not have index.
Index (Cat.# 30056): Each of our index primers contains a unique barcode sequence with 6 bases that can be used to identify libraries. Library multiplexing up to 48 samples is possible.
Unique dual index (Cat.# 30057): Library multiplexing up to 96 samples is possible with unique dual indexes. We have developed a 4-Base Difference Index System. The system allows us to make indexes that have at least 4 bases different from each other in the 8 bases index length. Our unique dual indexing primers remove sequencing errors such as index hopping, index cross-contamination, mis-assignment of reads, amplification errors, and de-multiplexing errors. The primer set includes 96 pre-mixed unique pairs of i5 and i7 index primers in a 96-well plate