Since SARS and MERS coronaviruses are pathogenic, research on them requires bio-safety level-3 procedures which can be difficult to carry out in most commercial and university laboratories. An alternative approach is to pseudotype lentiviral particles with the coronavirus coat proteins. This enables researchers to carry out studies on these viral elements in S2 laboratories.
We offer third-generation replication-incompetent lentiviral vectors expressing the tagGFP2 (ex. 483 nm, em. 506 nm) reporter gene, packaged as viral particles which are pseudotyped with the SARS-COV-2 D614G spike protein (S-protein) mutation, which shows enhanced recognition and binding to the ACE2 cell surface receptor to mediate coronavirus cell entry. Functional titration was performed in Cellecta HeLa-ACE2 Overexpression Cells.
This product enables investigators to run neutralization assays and related studies that investigate factors which influence D614G spike protein recognition and binding to target cells in S2 laboratories.
Contents: 50 ml SARS-CoV-2 D614G S-protein pseudotyped lentiviral particles, functionally titered by GFP fluorescence in HeLa-ACE2 overexpression cells: Titer > 10^4/ml.
Number of Reactions: 1000 rxns when using 50 ul reagent per well in a 96-well plate