TELL-Seq Microbial WGS Library Prep Kit, STD24

Description

Generate WGS libraries for small/microbial genome sizes up to 50 Mb

• 24 paired end TELL-Seq WGS libraries from samples with small genomes (1 Mb to 50 Mb)

This complete bundle kit includes the following components:

– Cat.# 110001: Microbial Library Reagent Box 1 (TELL-Seq enzymes and incubation buffers, storage at minus
20 C) and Microbial Library Reagent Box 2 (TELL-Seq barcoding beads, storage at plus 4 C).
– Cat.# 100003: TELL-Seq Library Multiplex Primers 1-8 (minus 20 C)
– Cat.# 100009: TELL-Seq Library Multiplex Primers 9-16 (minus 20 C)
– Cat.# 100010: TELL-Seq Library Multiplex Primers 17-24 (minus 20 C)
– Cat.# 100004: TELL-Seq Illumina Sequencing Primers (minus 20 C)

All components can also be ordered individually.

TELL-Seq: Long-Read Sequencing for All

• >50 kb sequence length on Illumina by barcode linked reads
• Ultralow DNA Input: 0.5 to 5 ng depending on genome size
• Simple single tube protocol – sequencing ready library in 3 hours
• Easy to automate for high throughput applications
• TELL-Seq software packages and barcode whitelist available for performing data analysis yourself

The TELL-Seq Microbial WGS library prep kit uses the highly innovative Transposase Enzyme Linked Long-read Sequencing (TELL-Seq™) technology ( Chen et al.,Genome Research, June 2020 ) for preparing a paired-end library to generate barcode linked highly accurate reads from an Illumina® sequencing system. Coupled with TELL-Seq specific analysis software, the linked reads can be easily used for de novo genome assembly