TELL-Seq WGS Library Prep Kit, STD4

Description

Generate WGS libraries for any genome size from 50 Mb to 5 Gb

• 12 paired end TELL-Seq WGS libraries from samples with small genomes (50 Mb to 200 Mb) or
• 6 paired end TELL-Seq WGS libraries from samples with medium sized genomes (200 Mb to 1 Gb) or
• 4 paired end TELL-Seq WGS libraries from samples with large sized genomes (1 Gb to 5 Gb)

This complete bundle kit includes the following components:

– Cat.# 110003: WGS Library Reagent Box 1 (TELL-Seq enzymes and incubation buffers, storage at minus 20 C).
– and WGS Library Reagent Box 2 (TELL-Seq barcoding beads, storage at plus 4 C).
– Cat.# 100003, or 100009, or 100010: TELL-Seq Library Multiplex Primers 1-8, or 9-16, or 17-24 (minus 20 C)
– Cat.# 100004: TELL-Seq Illumina Sequencing Primers (minus 20 C)

All components can also be ordered individually.

TELL-Seq: Long-Read Sequencing for All

• >50 kb sequence length on Illumina by barcode linked reads
• Ultralow DNA Input: 0.5 to 5 ng depending on genome size
• Simple single tube protocol – sequencing ready library in 3 hours
• Easy to automate for high throughput applications
• TELL-Seq software packages and barcode whitelist available for performing data analysis yourself

The TELL-Seq WGS library prep kit uses the highly innovative Transposase Enzyme Linked Long-read Sequencing (TELL-Seq™) technology ( Chen et al.,Genome Research, June 2020 ) for preparing a paired-end library to generate barcode linked highly accurate reads from an Illumina® sequencing system. Coupled with TELL-Seq specific analysis software, the linked reads can be easily used for the following applications and more:

• Genome wide variant calling
• Whole genome haplotype phasing
• Structural variation / Copy Number variation (CNV) detection
• Metagenomic studies: Deciphering microbial genomes in complex mixed samples
• de novo genome assembly