Description
The CleanPlex TMB 500 NGS Panel for Illumina® is a multiplex PCR-based targeted resequencing assay designed to enable rapid and accurate assessment of tumor mutational burden and mutation profile. The panel is expertly curated using the latest research findings to target 516 genes associated with major solid tumor types and maintains >96% correlation with whole exome datasets.
• Comprehensive Content -Interrogate 516 genes associated with the major solid tumor types to assess tumor mutational burden and characterize mutation profile
• Fast, Streamlined Workflow – Generate sequencing-ready libraries in just 6 hours using a rapid, three-step protocol
• Excellent Correlation with Whole Exome Sequencing – Prepare high-quality targeted NGS libraries using CleanPlex® Technology to replace the costly and time-consuming workflow of hybridization-based target enrichment
The CleanPlex® TMB 500 Panel is a targeted resequencing assay designed to enable rapid and accurate assessment of tumor mutational burden and comprehensive cancer profiling. The panel is expertly curated using the latest research findings to target 516 genes associated with major solid tumor types and maintains >96% correlation with whole exome datasets. The panel covers 1.64 Mb of genomic content (1.19 Mb exonic) and targets both single nucleotide variants (SNVs) and insertion-deletion mutations (indels). Two pools of multiplex PCR primers are utilized to construct target-enriched libraries from either formalin-fixed paraffin-embedded (FFPE) DNA from tumor samples or high-quality gDNA from blood samples. Starting with just 20 ng of DNA (10 ng per primer pool), sequencing-ready libraries can be prepared using a streamlined workflow in just 6 hours, allowing samples to be sequenced on the same day.
Kit Contents: Multiplex PCR Primers and PCR Reagents, & CleanPlex Targeted NGS Library Preparation Kit with CleanPlex purification reagents
Additional Products which can be ordered separately:
CleanMag® Magnetic Beads – for library purification and size selection
CleanPlex® Dual-Indexed PCR Primers – for multiplexing up to 2688 samples per sequencing run
CleanPlex® Unique Dual-Indexed PCR Primers – for low variant calling & other in-depth sequencing applications, and for ultra high multiplexing: each flow cell lane can multiplex up to 384 samples at once; with the multiple flow cell capability on a NovaSeq™ 6000, one can sequence up to 3,072 samples simultaneously for faster turn around and higher throughput workflows.