The CleanPlex® Unique Dual-Indexed (UDI) PCR Primers for Illumina® are high-quality ready-to-use PCR primers for Illumina library construction. They are compatible and designed for use with all CleanPlex Panels to construct targeted libraries for sequencing on an Illumina NGS platform. The index set comprises of uniquely pooled i5 and i7 index primer pools for unique dual multiplexing of samples per sequencing run. Each set of UDI primer pool only contains one instance of each i5 and each i7 to mitigate index hopping concerned on Illumina sequencing platforms with patterned flowcells. Samples indexes with each i5 and i7 pair can be pooled and sequenced then demultiplexed for analysis.
Contents: Set A includes 16 tubes of unique index pairs
Sets A and B’s index sequences are mutually exclusive and can be combined for a total of 32 available UDI options.