The CleanPlex UMI Lung Cancer NGS Panel for Illumina® is a multiplex PCR-based targeted resequencing assay designed for rapid and high confident detection of ultralow frequency variants across the hotspot regions of 23 genes associated with lung cancer.
• Relevant Gene Content – Interrogate 23 genes containing variants that are frequently mutated in lung cancer using a hotspot panel designed to work with cell-free DNA (cfDNA) and DNA isolated from FFPE
• Fast, Single-Tube Workflow – Generate high-quality molecular-barcoded and target-enriched NGS libraries in just 3.5 hours using a three-step, single-tube protocol
• Enhanced Error Correction – Remove PCR and sequencing errors with powerful error correction enabled by novel unique molecular identifiers (UMIs) that can distinguish the two strands of DNA
• High-Confidence Variant Detection – Detect low-frequency variants below 0.5% allele frequency with high specificity using just 30 ng of DNA
The CleanPlex® UMI Lung Cancer Panel is a targeted resequencing assay designed for rapid and high confident detection of low-frequency variants across the hotspot regions of 23 genes associated with lung cancer. Based on CleanPlex UMI technology, this panel is specifically designed to work with cell-free DNA (cfDNA) and FFPE DNA for precise analysis of tumor DNA in liquid biopsy and tumor profiling applications. The panel is powered by Paragon Genomics’ CleanPlex UMI Technology which incorporates novel unique molecular identifiers (UMIs) to enable correction of PCR and sequencing errors. Starting with just 20 ng of DNA, sequencing-ready libraries can be prepared using a single-tube workflow in just 3.5 hours. Low-frequency variants below 0.5% allele frequency can be confidently detected with high specificity using just 30 ng of DNA.
Kit Contents: Multiplex UMI PCR Primers and PCR Reagents, & CleanPlex UMI Targeted NGS Library Preparation Kit
Additional Products which can be ordered separately:
CleanMag® Magnetic Beads – for library purification and size selection
CleanPlex® Unique Dual-Indexed PCR Primers