• Single library with ~55,000 constructs
• Each gene is covered by 8 shRNAs targeting functional domains and conserved transcript regions
• Disease related genes covered—over 6,000 gene targets
Module 2 of Cellecta´s Human Genome-Wide (hGW) shRNA Library targeting ca. 6,300 genes, including many disease-associated genes not included in Module 1 of the library. Cellecta´s 3-module pooled lentiviral Human Genome-wide shRNA Library cloned in pRSI16cb targets nearly all protein-encoding genes of the human genome, and each gene is targeted by 8 hairpins. The library includes shRNA-specific barcodes that provide an alternative to direct sequencing of the shRNA target sequence which is difficult to sequence due to the stem-loop structure.
The library is available in either plasmid or lentiviral packaged formats.